The Squamosa Promoter Binding Protein (SBP) family comprises plant-specific transcription factors widely distributed across green plant lineages and holds significant value for agricultural improvement by regulating key traits including yield, flowering, and stress tolerance. Here, we performed a genome-wide analysis of Medicago truncatula and identified 22 SBP-box genes (MtSBP). These genes are irregularly distributed across seven chromosomes, with chromosome 6 devoid of any SBP-box gene. Sequence alignment confirmed that all MtSBP proteins share conserved nuclear localization signals and two zinc finger structures essential for transcriptional regulation. Phylogenetic analysis grouped thes... More >