Research Article
Open Access
A Novel Huperzine A-producing Endophytic Fungus NWUHS002 Isolated from Huperzia Serrata Trev.
1
College of Life Science, Northwest University, Xi’an 710069, China
2
Key Laboratory of Biotechnology of Shannxi Province, Xi’an 710069, China
3
Key Laboratory of Resource Biology and Biothchnology in Western China (Ministry of Education), Xi’an 710069, China
* Corresponding Author:
Bin Guo,
[email protected]
Volume 1, Issue 1
2024
Received: 27 September 2024
Accepted: 25 October 2024
Published: 08 November 2024
Article Information
Published in
Agricultural Science and Food Processing
Volume/Issue
Volume 1, Issue 1, 2024
Pages
21-28
Abstract
Huperzine A is an important drug for Alzheimer’s disease (AD) treatment, which is mainly extracted from the medicinal plant Huperzia serrata (Thunb.) Trev (Huperzia serrata Trev.). In present study, a new endophytic fungus (NWUHS002) producing Huperzine A was isolated from the Huperzia serrata Trev. with a yield of 5.20 µg/g. NWUHS002 was identified as Staphylotrichum by morphology and internal transcribed spacer (ITS) sequence. The discovery of NWUHS002 in Huperzia serrata Trev. can be used for the breeding of high-producing Huperzine A strains and the study of Huperzine A biosynthesis.
Graphical Abstract
Keywords
huperzine A
alzheimer’s disease
Huperzia serrata Trev.
endophyte
Funding
This work was supported by the Natural Science Basis Research Plan in Shaanxi Province of China under Grant 2023-JC-YB-165, Key Research and Development Plan Project of Shaanxi Province under Grant 2018ZDXM-SF-016, Xi’an Beilin District Science and Technology Plan Project under Grant GX2346 and Shaanxi Institute of Basic Sciences Project under Grant 23JHQ056.
References
- Vermunt, L., Sikkes, S. A., Van Den Hout, A., Handels, R., Bos, I., Van Der Flier, W. M., ... & AIBL Research Group. (2019). Duration of preclinical, prodromal, and dementia stages of Alzheimer’s disease in relation to age, sex, and APOE genotype. Alzheimer’s & Dementia, 15(7), 888-898.
[Google Scholar] - Alzheimer’s Association. (2019). 2019 Alzheimer’s disease facts and figures. Alzheimer’s & Dementia, 15(3), 321-387.
[Google Scholar] - World Health Organization. (2017). Global action plan on the public health response to dementia 2017–2025. World Health Organization.
[Google Scholar] - Ashraf, G. M., & Alexiou, A. (2019). Biological, Diagnostic and Therapeutic Advances in Alzheimer’s Disease. Springer Singapore.
[Google Scholar] - Roy, K. (Ed.). (2023). Computational modeling of drugs against Alzheimer’s disease (Vol. 203). Springer Nature.
[Google Scholar] - Ma, X., Tan, C., Zhu, D., Gang, D. R., & Xiao, P. (2007). Huperzine A from Huperzia species—An ethnopharmacolgical review. Journal of ethnopharmacology, 113(1), 15-34.
[Google Scholar] - Peng, Y., Jiang, L., Lee, D. Y., Schachter, S. C., Ma, Z., & Lemere, C. A. (2006). Effects of huperzine A on amyloid precursor protein processing and β-amyloid generation in human embryonic kidney 293 APP Swedish mutant cells. Journal of neuroscience research, 84(4), 903-911.
[Google Scholar] - Andrade, S., Ramalho, M. J., Loureiro, J. A., & Pereira, M. D. C. (2019). Natural compounds for Alzheimer’s disease therapy: a systematic review of preclinical and clinical studies. International journal of molecular sciences, 20(9), 2313.
[Google Scholar] - Yuan, H., Ma, Q., Ye, L., & Piao, G. (2016). The traditional medicine and modern medicine from natural products. Molecules, 21(5), 559.
[Google Scholar] - Ma, X., & Gang, D. R. (2008). In vitro production of huperzine A, a promising drug candidate for Alzheimer’s disease. Phytochemistry, 69(10), 2022-2028.
[Google Scholar] - Ferreira, A., Rodrigues, M., Fortuna, A., Falcão, A., & Alves, G. (2016). Huperzine A from Huperzia serrata: a review of its sources, chemistry, pharmacology and toxicology. Phytochemistry reviews, 15, 51-85.
[Google Scholar] - White, J. D., Li, Y., Kim, J., & Terinek, M. (2013). A Novel Synthesis of (−)-Huperzine A via Tandem Intramolecular Aza-Prins Cyclization–Cyclobutane Fragmentation. Organic letters, 15(4), 882-885.
[Google Scholar] - Flores-Bustamante, Z. R., Rivera-Orduna, F. N., Martínez-Cárdenas, A., & Flores-Cotera, L. B. (2010). Microbial paclitaxel: advances and perspectives. The Journal of antibiotics, 63(8), 460-467.
[Google Scholar] - Sang, X., Yang, M., & Su, J. (2020). Research on endophytic fungi for producing huperzine A on a large-scale. Critical Reviews in Microbiology, 46(6), 654-664.
[Google Scholar] - Ellman, G. L., Courtney, K. D., Andres Jr, V., & Featherstone, R. M. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical pharmacology, 7(2), 88-95.
[Google Scholar] - Felsenstein, J. (1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39(4), 783-791.
[Google Scholar] - Munro, H. N. (Ed.). (2012). Mammalian protein metabolism (Vol. 4). Elsevier.
[Google Scholar] - Saitou, N., & Nei, M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular biology and evolution, 4(4), 406-425.
[Google Scholar] - Kumar, S., Stecher, G., & Tamura, K. (2016). MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular biology and evolution, 33(7), 1870-1874.
[Google Scholar] - Cruz-Miranda, O. L., Folch-Mallol, J., Martínez-Morales, F., Gesto-Borroto, R., Villarreal, M. L., & Taketa, A. C. (2020). Identification of a Huperzine A-producing endophytic fungus from Phlegmariurus taxifolius. Molecular biology reports, 47(1), 489-495.
[Google Scholar] - Dong, L. H., Fan, S. W., Ling, Q. Z., Huang, B. B., & Wei, Z. J. (2014). Indentification of huperzine A-producing endophytic fungi isolated from Huperzia serrata. World Journal of Microbiology and Biotechnology, 30, 1011-1017.
[Google Scholar] - JU, Z., WANG, J., & PAN, S. (2009). Isolation and preliminary identification of the endophytic fungi which produce Hupzine A from four species in Hupziaceae and determination of Huperzine A by HPLC. Fudan University Journal of Medical Sciences, 445-449.
[Google Scholar] - Kang, X., Liu, C., Shen, P., Hu, L., Lin, R., Ling, J., ... & Liu, D. (2019). Genomic characterization provides new insights into the biosynthesis of the secondary metabolite huperzine a in the endophyte Colletotrichum gloeosporioides Cg01. Frontiers in microbiology, 9, 3237.
[Google Scholar] - Le, T. T. M., Hoang, A. T. H., Nguyen, N. P., Le, T. T. B., Trinh, H. T. T., Vo, T. T. B., & Quyen, D. V. (2020). A novel huperzine A-producing endophytic fungus Fusarium sp. Rsp5. 2 isolated from Huperzia serrate. Biotechnology letters, 42(6), 987-995.
[Google Scholar] - Li, W. K., Zhou, J. Y., Lin, Z. W., & Hu, Z. B. (2007). Study on fermentation condition for production of huperzine A from endophytic fungus 2F09P03B of Huperzia serrata. Chin Med Biotechnol, 2(4), 254-259.
[Google Scholar] - Shu, S., Zhao, X., Wang, W., Zhang, G., Cosoveanu, A., Ahn, Y., & Wang, M. (2014). Identification of a novel endophytic fungus from Huperzia serrata which produces huperzine A. World Journal of Microbiology and Biotechnology, 30, 3101-3109.
[Google Scholar] - Su, J., & Yang, M. (2015). Huperzine A production by Paecilomyces tenuis YS-13, an endophytic fungus isolated from Huperzia serrata. Natural Product Research, 29(11), 1035-1041.
[Google Scholar] - Wen-Xia, H., Zhong-Wen, H., Min, J., Han, Z., Wei-Ze, L., Li-Bin, Y., ... & Xiao-Feng, L. (2020). Five novel and highly efficient endophytic fungi isolated from Huperzia serrata expressing huperzine A for the treatment of Alzheimer’s disease. Applied Microbiologyand Biotechnology, 104, 9159-9177.
[Google Scholar] - Yang, X., Guo, H., Gao, X., Gao, F., & Wang, L. (2006). Studies on the secondary metabolism of endophytes YD-01 I. JOURNAL of CHINA PHARMACEUTICAL UNIVERSITY, 37(5), 479.
[Google Scholar] - Zhang, Z. B., Zeng, Q. G., Yan, R. M., Wang, Y., Zou, Z. R., & Zhu, D. (2011). Endophytic fungus Cladosporium cladosporioides LF70 from Huperzia serrata produces Huperzine A. World Journal of Microbiology and Biotechnology, 27, 479-486.
[Google Scholar] - Zhu, D., Wang, J., Zeng, Q., Zhang, Z., & Yan, R. (2010). A novel endophytic Huperzine A–producing fungus, Shiraia sp. Slf14, isolated from Huperzia serrata. Journal of applied microbiology, 109(4), 1469-1478.
[Google Scholar]
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APA Style
Liu, R., Zhao, Y., Wang, H., Zhang, H., Yue, X., Guo, H., Luo, J., & Guo, B. (2024). A Novel Huperzine A-producing Endophytic Fungus NWUHS002 Isolated from Huperzia Serrata Trev.. Agricultural Science and Food Processing, 1(1), 21–28. https://doi.org/10.62762/ASFP.2024.857122
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TY - JOUR AU - Liu, Rongxing AU - Zhao, Yirui AU - Wang, He AU - Zhang, Hao AU - Yue, Xizhen AU - Guo, Hongwei AU - Luo, Jinmei AU - Guo, Bin PY - 2024 DA - 2024/11/08 TI - A Novel Huperzine A-producing Endophytic Fungus NWUHS002 Isolated from Huperzia Serrata Trev. JO - Agricultural Science and Food Processing T2 - Agricultural Science and Food Processing JF - Agricultural Science and Food Processing VL - 1 IS - 1 SP - 21 EP - 28 DO - 10.62762/ASFP.2024.857122 UR - https://www.icck.org/article/abs/ASFP.2024.857122 KW - huperzine A KW - alzheimer’s disease KW - Huperzia serrata Trev. KW - endophyte AB - Huperzine A is an important drug for Alzheimer’s disease (AD) treatment, which is mainly extracted from the medicinal plant Huperzia serrata (Thunb.) Trev (Huperzia serrata Trev.). In present study, a new endophytic fungus (NWUHS002) producing Huperzine A was isolated from the Huperzia serrata Trev. with a yield of 5.20 µg/g. NWUHS002 was identified as Staphylotrichum by morphology and internal transcribed spacer (ITS) sequence. The discovery of NWUHS002 in Huperzia serrata Trev. can be used for the breeding of high-producing Huperzine A strains and the study of Huperzine A biosynthesis. SN - 3066-1579 PB - Institute of Central Computation and Knowledge LA - English ER -
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@article{Liu2024A,
author = {Rongxing Liu and Yirui Zhao and He Wang and Hao Zhang and Xizhen Yue and Hongwei Guo and Jinmei Luo and Bin Guo},
title = {A Novel Huperzine A-producing Endophytic Fungus NWUHS002 Isolated from Huperzia Serrata Trev.},
journal = {Agricultural Science and Food Processing},
year = {2024},
volume = {1},
number = {1},
pages = {21-28},
doi = {10.62762/ASFP.2024.857122},
url = {https://www.icck.org/article/abs/ASFP.2024.857122},
abstract = {Huperzine A is an important drug for Alzheimer’s disease (AD) treatment, which is mainly extracted from the medicinal plant Huperzia serrata (Thunb.) Trev (Huperzia serrata Trev.). In present study, a new endophytic fungus (NWUHS002) producing Huperzine A was isolated from the Huperzia serrata Trev. with a yield of 5.20 µg/g. NWUHS002 was identified as Staphylotrichum by morphology and internal transcribed spacer (ITS) sequence. The discovery of NWUHS002 in Huperzia serrata Trev. can be used for the breeding of high-producing Huperzine A strains and the study of Huperzine A biosynthesis.},
keywords = {huperzine A, alzheimer’s disease, Huperzia serrata Trev., endophyte},
issn = {3066-1579},
publisher = {Institute of Central Computation and Knowledge}
}
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Copyright © 2024 by the Author(s). Published by Institute of Central Computation and Knowledge. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.
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