Obligate crassulacean acid metabolism (CAM) plants survive karst drought through daytime stomatal closure, which limits water loss but suspends gas exchange, raising the question of how intracellular metabolism continues under closed stomata. Using a reintroduced population of the orchid Dendrobium loddigesii shaped by 15 years of natural selection, we combined a two-season (January and April) between-population comparison with concurrent within-plant comparisons of different-aged leaves, and quantified leaf electrophysiology using an extended Hodgkin-Huxley model. Intracellular water transport rate (WTR) was highly conserved across seasons and leaf ages (overall mean 51.25, within-group CV... More >

Hailstorms cause severe mechanical damage to crops, yet their long-term legacy effects on the physiology of perennial medicinal herbs remain poorly understood. This study examined \textit{Bletilla striata} plants damaged by a severe hailstorm on April 2, 2026. On day 37 post-damage, coinciding with new pseudobulb formation, 44 electrophysiological parameters were measured in leaves of damaged plants ($n = 8$) and undamaged controls ($n = 8$) and compared using Welch's $t$-test. Damaged leaves showed a persistent ``low-resistance, high-capacitance'' pattern: inherent physiological resistance decreased to 25.0% of the control value, whereas inherent capacitance increased 5.6-fold (both $P < 0.... More >

Plant electrophysiology is undergoing a profound paradigm shift from traditional phenomenological observation to systemic signal decoding, with mature methodologies from computational neuroscience and brain-computer interface technologies providing critical theoretical and engineering support for this interdisciplinary evolution. This review first systematically summarizes the evolution of flexible wearable electrodes and ultra-high impedance amplification hardware systems tailored to the ultra-slow signal dynamics and continuous morphological growth characteristics of plants. Second, we discuss the application pathways of introducing standardized sequential evoked paradigms from neuroscienc... More >

Enshi is a high-incidence area for Plasmodiophora brassicae infection in Brassica rapa in China; however, the differential immunomodulatory effects of selenite (Se\(^{4+}\)) on the infection of Brassica rapa by the Plasmodiophora brassicae (Pb) remain unclear. In this study, Enshi Gaoshan Brassica rapa (GBr) was selected. Pre-treatment with 0–10 mg/L Se\(^{4+}\) was applied, followed by artificial inoculation with Plasmodiophora brassicae spores (8×10\(^9\) spore/mL). Growth characteristics, photosynthesis, and electrophysiological and nutritional metabolic features of GBr leaves were measured. The results indicate that electrophysiological techniques provide more significant distinctions... More >

Up to date technologies, created by humans and utilizing electromagnetic fields (EMFs), present both anticipated benefits and undesirable side effects. These effects can influence the living tissues of all exposed biodiversity, in accordance with the "One Health" principle. The operation of modern natural parks encourages Internet connections via antennas, linked to park management, security, and telecommunications. These connectivity needs are tied to the functioning of all living organisms within the park, which depend on environmental conditions, according to the time and season. The antenna providing Internet access is a source of EMF; this coverage/exposure relationship can be monitored... More >

Photosynthesis is the fundamental biochemical process driving Earth's biogeochemical cycles. The traditional theory holds that water is the sole source of photosynthetic oxygen and atmospheric CO$_2$ the only inorganic carbon substrate. However, electrophysiological, isotopic, and physiological evidence from our systematic research prompts a re-examination of this paradigm, revealing an unrecognized coupling between plant inorganic carbon assimilation and intracellular water utilization. Our key findings are: (i) Intracellular water utilization rate is decoupled from atmospheric CO$_2$ assimilation, suggesting that terrestrial plants assimilate inorganic carbon from atmospheric CO$_2$, soil... More >

The conservation of rare and endangered medicinal plants, such as Cremastra appendiculata, is hindered by a limited understanding of their intrinsic endangerment mechanisms. This study employed a plant vitality analyzer (model: ZT-FIA-1, Jiangsu Zhongtian Zhigan Life Data Co., Ltd., China) to systematically measure 50 electrophysiological kinetic parameters in the leaves of C. appendiculata and the fast-growing reference species Isatis tinctoria. Parameters encompassed water metabolism, nutrient transport, dielectric substance translocation, energy metabolism, and comprehensive stress resistance. Compared with I. tinctoria, C. appendiculata exhibited significantly higher intrinsic resistance... More >