Plant electrophysiology is undergoing a profound paradigm shift from traditional phenomenological observation to systemic signal decoding, with mature methodologies from computational neuroscience and brain-computer interface technologies providing critical theoretical and engineering support for this interdisciplinary evolution. This review first systematically summarizes the evolution of flexible wearable electrodes and ultra-high impedance amplification hardware systems tailored to the ultra-slow signal dynamics and continuous morphological growth characteristics of plants. Second, we discuss the application pathways of introducing standardized sequential evoked paradigms from neuroscienc... More >

Enshi is a high-incidence area for Plasmodiophora brassicae infection in Brassica rapa in China; however, the differential immunomodulatory effects of selenite (Se\(^{4+}\)) on the infection of Brassica rapa by the Plasmodiophora brassicae (Pb) remain unclear. In this study, Enshi Gaoshan Brassica rapa (GBr) was selected. Pre-treatment with 0–10 mg/L Se\(^{4+}\) was applied, followed by artificial inoculation with Plasmodiophora brassicae spores (8×10\(^9\) spore/mL). Growth characteristics, photosynthesis, and electrophysiological and nutritional metabolic features of GBr leaves were measured. The results indicate that electrophysiological techniques provide more significant distinctions... More >

Up to date technologies, created by humans and utilizing electromagnetic fields (EMFs), present both anticipated benefits and undesirable side effects. These effects can influence the living tissues of all exposed biodiversity, in accordance with the "One Health" principle. The operation of modern natural parks encourages Internet connections via antennas, linked to park management, security, and telecommunications. These connectivity needs are tied to the functioning of all living organisms within the park, which depend on environmental conditions, according to the time and season. The antenna providing Internet access is a source of EMF; this coverage/exposure relationship can be monitored... More >

Photosynthesis is the fundamental biochemical process driving Earth's biogeochemical cycles. The traditional theory holds that water is the sole source of photosynthetic oxygen and atmospheric CO$_2$ the only inorganic carbon substrate. However, electrophysiological, isotopic, and physiological evidence from our systematic research prompts a re-examination of this paradigm, revealing an unrecognized coupling between plant inorganic carbon assimilation and intracellular water utilization. Our key findings are: (i) Intracellular water utilization rate is decoupled from atmospheric CO$_2$ assimilation, suggesting that terrestrial plants assimilate inorganic carbon from atmospheric CO$_2$, soil... More >

The conservation of rare and endangered medicinal plants, such as Cremastra appendiculata, is hindered by a limited understanding of their intrinsic endangerment mechanisms. This study employed a plant vitality analyzer (model: ZT-FIA-1, Jiangsu Zhongtian Zhigan Life Data Co., Ltd., China) to systematically measure 50 electrophysiological kinetic parameters in the leaves of C. appendiculata and the fast-growing reference species Isatis tinctoria. Parameters encompassed water metabolism, nutrient transport, dielectric substance translocation, energy metabolism, and comprehensive stress resistance. Compared with I. tinctoria, C. appendiculata exhibited significantly higher intrinsic resistance... More >

Plants, being sessile, must constantly monitor and respond to environmental fluctuations and stressors. Among the diverse signalling modalities (chemical, hydraulic, hormonal), electrical signals stand out for their rapid propagation and capacity to serve as early warning cues. In this review, we examine the concept of electrobiological signatures—distinctive patterns of electrical activity in plants as potential early indicators of stress and adaptation. This review first discusses the fundamental mechanisms such as ion fluxes, membrane transporters, coupling with calcium dynamics, reactive oxygen species (ROS), and cross-talk with hormonal networks. Next, it explores how these signatures... More >

Investigating the intracellular water dynamics within leaves will clarify plant responses to different water conditions and provide a theoretical basis for enhancing water-use efficiency. Detached leaves of Chinese flowering cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) and broccoli (Brassica oleracea L. var. italica Plenck) were used as experimental materials in this study. Electrophysiological parameters of the leaves under saturated and non-saturated conditions were measured, and the intracellular water, nutrient, and energy related indices were calculated according to Nernst equation. The acceleration (a) reflecting the change rate of water transport rate in cel... More >

An individual plant cell functions as a complex dynamic circuit, composed of resistor, capacitor, and inductor networks created by cellular structures and macromolecules. Furthermore, high-voltage electrostatic fields (HVEF) alter its electrical properties and initiate metabolic changes. This study aimed to examine the impact of HVEF on the kinetic properties of potassium (K$^+$) and phosphate (H$_2$PO$_4^-$) absorption by Cucumis sativus L. seedlings, and further probed the regulatory mechanisms by constructing a cell membrane potential model. Experimental findings revealed that HVEF substantially altered the maximum uptake rate ($I_{max}$), half-saturation constant ($K_m$), and minimum cr... More >