Evaluation of the Compound-microbes-preparation Based on Microflora Information as Inoculants in the Silage

Xuhui Zhuang; Wei Han; Ziyu Xue; Haijiang Miao

doi:10.62762/ASFP.2024.516036

Volume 1, Issue 1, Agricultural Science and Food Processing

Volume 1, Issue 1, 2024

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Jie Ouyang

Beijing Forestry University, China

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Agricultural Science and Food Processing, Volume 1, Issue 1, 2024: 29-37

Open Access | Research Article | 10 November 2024

Evaluation of the Compound-microbes-preparation Based on Microflora Information as Inoculants in the Silage

Xuhui Zhuang 1

Wei Han 1 *

Ziyu Xue 1

Haijiang Miao 1

1 Academy of State Administration of Grain, Beijing 100037, China

* Corresponding Author: Wei Han, [email protected]

DOI: 10.62762/ASFP.2024.516036

ARK: ark:/57805/asfp.2024.516036

Received: 19 September 2024, Accepted: 21 October 2024, Published: 10 November 2024

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Abstract

Ensiling technique is becoming a common way to preserve fresh corn and grass. This study aimed at evaluating the silages’ quality with a new type of inoculants designed based on the microflora information of the native silages. A total of 33 silage samples were analyzed for their microbial community structure by high throughput sequencing. A total of 73 dominant strains were isolated with a clear goal. The corn was ensiled by dominant strains (Lactobacillus buchneri II JC1-5: Acetobacter pasteurianus DHL-8 = 4:3; 109 CFU/g) as inoculants. In a 15-day trial period, the aerobic stability of this inoculated silage was significantly higher than the control group. There was a positive correlation between lactic acid content and pH/mold number (R2 values of 0.64331 and 0.48584, respectively). Compared to the control group, ammonia nitrogen (NH3-N), water soluble carbohydrates (WSC), neutral detergent fiber (NDF), and acid detergent fiber (ADF) decreased by 57.7%, 12.5%, 1.4%, and 7.5%, respectively; crude protein (CP) increased by 1.1%. Our results indicate that the new type of inoculants can improve the quality of the silage.

Graphical Abstract

Keywords

silage

inoculants

microflora

high throughput sequencing

Funding

This work was supported without any funding.

References

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Zhuang, X., Han, W., Xue, Z., & Miao, H. (2024). Evaluation of the Compound-microbes-preparation Based on Microflora Information as Inoculants in the Silage. Agricultural Science and Food Processing, 1(1), 29–37. https://doi.org/10.62762/ASFP.2024.516036

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TY  - JOUR
AU  - Zhuang, Xuhui
AU  - Han, Wei
AU  - Xue, Ziyu
AU  - Miao, Haijiang
PY  - 2024
DA  - 2024/11/10
TI  - Evaluation of the Compound-microbes-preparation Based on Microflora Information as Inoculants in the Silage
JO  - Agricultural Science and Food Processing
T2  - Agricultural Science and Food Processing
JF  - Agricultural Science and Food Processing
VL  - 1
IS  - 1
SP  - 29
EP  - 37
DO  - 10.62762/ASFP.2024.516036
UR  - https://www.icck.org/article/abs/ASFP.2024.516036
KW  - silage
KW  - inoculants
KW  - microflora
KW  - high throughput sequencing
AB  - Ensiling technique is becoming a common way to preserve fresh corn and grass. This study aimed at evaluating the silages’ quality with a new type of inoculants designed based on the microflora information of the native silages. A total of 33 silage samples were analyzed for their microbial community structure by high throughput sequencing. A total of 73 dominant strains were isolated with a clear goal. The corn was ensiled by dominant strains (Lactobacillus buchneri II JC1-5: Acetobacter pasteurianus DHL-8 = 4:3; 109 CFU/g) as inoculants. In a 15-day trial period, the aerobic stability of this inoculated silage was significantly higher than the control group. There was a positive correlation between lactic acid content and pH/mold number (R2 values of 0.64331 and 0.48584, respectively). Compared to the control group, ammonia nitrogen (NH3-N), water soluble carbohydrates (WSC), neutral detergent fiber (NDF), and acid detergent fiber (ADF) decreased by 57.7%, 12.5%, 1.4%, and 7.5%, respectively; crude protein (CP) increased by 1.1%. Our results indicate that the new type of inoculants can improve the quality of the silage.
SN  - 3066-1579
PB  - Institute of Central Computation and Knowledge
LA  - English
ER  -

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@article{Zhuang2024Evaluation,
  author = {Xuhui Zhuang and Wei Han and Ziyu Xue and Haijiang Miao},
  title = {Evaluation of the Compound-microbes-preparation Based on Microflora Information as Inoculants in the Silage},
  journal = {Agricultural Science and Food Processing},
  year = {2024},
  volume = {1},
  number = {1},
  pages = {29-37},
  doi = {10.62762/ASFP.2024.516036},
  url = {https://www.icck.org/article/abs/ASFP.2024.516036},
  abstract = {Ensiling technique is becoming a common way to preserve fresh corn and grass. This study aimed at evaluating the silages’ quality with a new type of inoculants designed based on the microflora information of the native silages. A total of 33 silage samples were analyzed for their microbial community structure by high throughput sequencing. A total of 73 dominant strains were isolated with a clear goal. The corn was ensiled by dominant strains (Lactobacillus buchneri II JC1-5: Acetobacter pasteurianus DHL-8 = 4:3; 109 CFU/g) as inoculants. In a 15-day trial period, the aerobic stability of this inoculated silage was significantly higher than the control group. There was a positive correlation between lactic acid content and pH/mold number (R2 values of 0.64331 and 0.48584, respectively). Compared to the control group, ammonia nitrogen (NH3-N), water soluble carbohydrates (WSC), neutral detergent fiber (NDF), and acid detergent fiber (ADF) decreased by 57.7\%, 12.5\%, 1.4\%, and 7.5\%, respectively; crude protein (CP) increased by 1.1\%. Our results indicate that the new type of inoculants can improve the quality of the silage.},
  keywords = {silage, inoculants, microflora, high throughput sequencing},
  issn = {3066-1579},
  publisher = {Institute of Central Computation and Knowledge}
}

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